OPIOID RECPT DELTA; RB X-50UL

Analysis Note

ControlSpinal cord

Application

Research Sub CategoryNeuroinflammation & Pain

Research CategoryNeuroscience

Immunohistochemistry on CNS tissues fixed in PLP: 1:1,000.

Reactive on paraffin embedded tissue.

Western Blot: 1:1000. Under reducing conditions the antibody will detect major bands at 36 and 72 kDa.

FACS on human T-cells (Sharp, 2001)

Optimal working dilutions must be determined by the end user.

IMMUNOHISTOCHEMISTRY PROTOCOL FOR AB1560

This antibody has been used successfully on 30 mm, free floating, 4% paraformaldehyde fixed rat brain tissue. All steps are performed under constant agitation. Suggested protocol follows.

1) 3 x 10 minute washes in TBS (without Triton).

2) Incubate for 30 minutes in TBS with 3% serum (same as host from secondary antibody).

3) Incubate primary antibody diluted appropriately in TBS with 1% serum (same as host from secondary antibody) (without Triton) for 2 hours at room temperature followed by 16 hours at 4°C.

4) 3 x 10 minute washes in TBS.

5) Incubate with secondary antibody diluted appropriately in TBS with1% serum (same as host from secondary antibody).

6) 3 x 10 minute washes in TBS.

7) ABC Elite (1:200 Vector Labs) in TBS.

8) 2 x 10 minute washes in TBS.

9) 1 x 10 minute wash in phosphate buffer (no saline).

10) DAB reaction with 0.06% NiCl added for intensification.

11) 2 x 10 minute washes in PBS.

12) 1 x 10 minute wash in phosphate buffer (no saline).

Anti-Opioid Receptor Antibody, δ, pain, N-terminus detects level of Opioid Receptor & has been published & validated for use in FC, IH(P) & WB.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Delta opioid receptor N-terminal peptide (LVPSARAELQSSPLV).

Epitope: N-terminus

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Linkage

Replaces: MAB5330

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

UnPurified whole rabbit antiSerum containing no preservatives.

Unpurified

Specificity

Recognizes Delta opioid receptor. The control peptide (catalog number AG288) is available for use in antibody blocking studies.

Storage and Stability

Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Target description

36 and 72 kDa